Join us at the 13th annual PEGS Boston!

Going to PEGS Boston? Drop by booth #326 where we will be showcasing mirrorball

Venue: Seaport World Trade Center, Boston, MA, USA
Date: 1 – 5  May 2017
Booth: #326

We would like to invite you to the following workshop:
Title: High-Throughput Screening for Antibody Discovery Using mirrorball
Presenter: Christyne J. Kane, Senior Scientist, AbbVie Bioresearch Center, Inc.
Abstract:
The antibody discovery industry has begun to set aside traditional assay formats such as ELISA or flow cytometry in favor of more sensitive, high-throughput technologies to screen for novel biologic candidates. This presentation will highlight a selection of multiplex assays for the screening of hybridoma supernatants enabling the discovery process.

Meet mirrorball

TTP Labtech’s mirrorball high sensitivity fluorescence cytometer is a highly versatile instrument that brings process efficiencies to multiple stages in the biologics screening pipeline – from early screening and hit identification, through to characterisation and phenotypic cellular responses.

Key applications include:

  • biologics screening:
    – hybridoma, phage display, B-cell cloning, antibody fragments, peptides, scFv, Fab
  • binding characterisation:
    – Bmaxand Kd determination
  • functional assays:
    – cell health, cell cycle, proliferation, cell signalling
  • quantification of soluble proteins:
    – cytokines, hormones, growth factors

do more antibody screening with less

mirrorball represents a complete biologics screening solution. It offers analysis of bead-bound soluble antigens and cell-surface antigens expressed on both adherent and suspension cell lines in microplates. It is compatible both with washed and no-wash assay formats.

mirrorball’s multiplexing capabilities enable hit determination in high density microplates while simultaneously screening out non-specific and non-selective hits in the same well. This means antibody engineering researchers can identify cross-reactivity and selectivity but – significantly – without the loss of throughput.

Key benefits:

  • a streamlined no-wash assay format
  • unprecedented levels of throughput
  • maintaining cell morphology through in situ analysis of adherent cells
  • investigate multiple targets in one well
  • more stringent results by screening out non-specific and non-selective hits in the same sample
  • assay miniaturisation using high density microplates
  • visual confirmation of your results using an image for QC

Our team can also discuss with you our instruments used for:

  • flexible sample management workflows from ambient to -80°C (comPOUND, arktic, lab2lab)
  • unique low volume, positive displacement liquid handling for any liquid including cells or beads (mosquito, dragonfly).

Want to find out more? We offer a full range of resources, read our application notes and posters here…

application notes:

posters:

For more information about the PEGS Boston visit their website here.

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