Going to PEGS Boston? Drop by booth #326 where we will be showcasing mirrorball
Venue: Seaport World Trade Center, Boston, MA, USA
Date: 1 – 5 May 2017
We would like to invite you to the following workshop:
Title: High-Throughput Screening for Antibody Discovery Using mirrorball
Presenter: Christyne J. Kane, Senior Scientist, AbbVie Bioresearch Center, Inc.
The antibody discovery industry has begun to set aside traditional assay formats such as ELISA or flow cytometry in favor of more sensitive, high-throughput technologies to screen for novel biologic candidates. This presentation will highlight a selection of multiplex assays for the screening of hybridoma supernatants enabling the discovery process.
TTP Labtech’s mirrorball high sensitivity fluorescence cytometer is a highly versatile instrument that brings process efficiencies to multiple stages in the biologics screening pipeline – from early screening and hit identification, through to characterisation and phenotypic cellular responses.
Key applications include:
- biologics screening:
– hybridoma, phage display, B-cell cloning, antibody fragments, peptides, scFv, Fab
- binding characterisation:
– Bmaxand Kd determination
- functional assays:
– cell health, cell cycle, proliferation, cell signalling
- quantification of soluble proteins:
– cytokines, hormones, growth factors
do more antibody screening with less
mirrorball represents a complete biologics screening solution. It offers analysis of bead-bound soluble antigens and cell-surface antigens expressed on both adherent and suspension cell lines in microplates. It is compatible both with washed and no-wash assay formats.
mirrorball’s multiplexing capabilities enable hit determination in high density microplates while simultaneously screening out non-specific and non-selective hits in the same well. This means antibody engineering researchers can identify cross-reactivity and selectivity but – significantly – without the loss of throughput.
- a streamlined no-wash assay format
- unprecedented levels of throughput
- maintaining cell morphology through in situ analysis of adherent cells
- investigate multiple targets in one well
- more stringent results by screening out non-specific and non-selective hits in the same sample
- assay miniaturisation using high density microplates
- visual confirmation of your results using an image for QC
Our team can also discuss with you our instruments used for:
- flexible sample management workflows from ambient to -80°C (comPOUND, arktic, lab2lab)
- unique low volume, positive displacement liquid handling for any liquid including cells or beads (mosquito, dragonfly).
Want to find out more? We offer a full range of resources, read our application notes and posters here…
- mirroball: simple competition binding assays with mirrorball
- mirrorball: antibody screening against cell surface receptors (EGFRs)
- mirrorball: development of a homogenous RSV neutralisation assay
- mirrorball: enhanced ELISA workflows with no-wash multiplexed fluorescent bead assays
- mirrorball: improving the reliability of cell-based immunoassay screening through multiplexing with mirrorball
- mirrorball: multiplexed Fluorescence ELISA assays on mirrorball
- mirrorball: no-wash antibody titre determination on the mirrorball fluorescence cytometer
- mirrorball: simultaneous determination of antibody binding, specificity and titer on the mirrorball fluorescence cytometer
- mirrorball: cell multiplexing in homogeneous antibody binding assays
- mirrorball: development of a homogenous RSV neutralization assay on the mirrorball fluorescence cytometer
- mirrorball: homogeneous binding assay for hybridoma screening
- mirrorball: multiplexed cell- and bead-based assays in a no-wash format for biologics screening and characterisation
- mirrorball: simultaneous determination of antibody binding, specificity and titre on the mirrorball fluorescence cytometer
For more information about the PEGS Boston visit their website here.