16 - 18 Oct 2017 We will be at Single Cell Genomics 2017!

See you at Single Cell Genomics 2017 and discover how mosquito liquid handlers can  work in a wide range of genomics applications!

Venue: The David Lopatie Conference Centre, Weizmann Institute of Science, Israel
Date: 16 – 18 Oct 2017

The conference will bring together many of the pioneers and leading experts in the field to three days of extensive, interdisciplinary and informal discussion.

We will be showcasing our mosquito liquid handling robots (single, 8- or 16-channel; volume range 25 -1200 nL or 0.5 – 5 µL) which can be used for miniaturising a wide range of genomics applications:

  • Next-Generation Sequencing – Low-cost, low-volume library preparation, including magnetic bead clean-up and normalisation; validated with market leading kits including Illumina Nextera XT.
  • Single Cell Genomics – miniaturised cDNA synthesis, whole genome amplification and library prep.
  • Synthetic & molecular biology – High-throughput, miniaturised reactions for PCR, cloning and DNA assembly.
  • Gene expression profiling – Cost-effective, high-throughput analysis of mRNA expression with rapid qPCR set-up.

At final volumes of 5 μL or less, miniaturised workflows can reduce sample preparation costs by 90% or more, while providing increased throughput, process control and standardisation.

Find out how GeneMill (University of Liverpool) managed to increase their throughput of the standard synthetic biology workflow in our latest application note >> Get the app note here

For more information about the event, please click here

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3 - 5 Oct 2017 See mosquito HTS in action at SynBioBeta 2017!

We look forward to seeing you at SynBioBeta 2017

Venue: Mission Bay Conference Center, San Francisco, CA
Date: 3 – 5 Oct 2017

We hope that you will join us at SynBioBeta 2017 that will be held at Mission Bay Conference Center in San Francisco.

The conference this year includes: biomaterials, biosecurity and policy, conservation and the environment, consumer products, education and public outreach. Learn about emerging applications for DNA, health and drug discovery, reproducibility, investment opportunities, and more.

We will be showcasing out mosquito HTS, the cost-effective assay miniaturisation, multitalented liquid handler!

Application areas include:

  • serial dilutions
  • plate reformatting and replication
  • acoustic plate source creation
  • next generation sequencing
  • genotyping
  • high throughput MALDI-TOF MS

>> Read more about the benefits using mosquito HTS for these applications

For more information about the event please visit their website here

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alternative acoustic liquid handling tubes

labCrystal 2017

Press release: Abcam and TTP Labtech Collaborate to Develop sol-R Reagent Kits

Press release: Abcam and TTP Labtech Collaborate to Develop sol-R Reagent Kits

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Assay-ready kits will incorporate fully-validated, industry-leading recombinant antibody technology for high throughput research

Cambridge, UK, 16th August 2017: TTP Labtech Ltd, a global leader in the design and development of automated instrumentation and consumables for life science applications, and Abcam plc, a global  innovator in life sciences reagents and tools, today announced a collaboration to further develop reagent kits for the quantification of secreted proteins in multiplexed screening assays.

Following the successful launch last year of sol-R coded beads, TTP Labtech is working with Abcam to meet customer needs by developing assay ready kits which enable researchers to deliver data driven decisions faster and with confidence. The sol-R reagent kits will offer Abcam’s highly validated and quality assured antibodies including recombinant antibody pairs, combined with the reliability of sol-R coded beads and mirrorball® fluorescence cytometry technology from TTP Labtech for sample analysis.

Joby Jenkins, director – product strategy, TTP Labtech, commented: “Our sol-R coded beads are designed for secreted protein quantification in multiplexed no-wash immunoassay screens. Working with Abcam on the development of the sol-R reagent kits enables us to combine their advanced antibody technology and reagent expertise with our sol-R bead technology to provide convenient off-the-shelf kits. Since the kits are validated on TTP Labtech’s mirrorball plate-based fluorescent cytometer, repeated wash steps associated with traditional sandwich ELISA’s are no longer necessary, thereby significantly reducing assay times, while delivering data integrity.”

John Baker, Senior Vice President Portfolio and Business Development, at Abcam, said: “The integration of Abcam’s high quality antibody technology with
TTP Labtech’s sol-R Reagents will deliver reproducibility, quality and efficiency to life science researchers, accelerating their research. The new kits have been designed for researchers carrying out high-throughput immunoassays including drug discovery in biotech, pharma and contract research organisations.”

TTP Labtech will offer Alexa Fluor® technology1, one of the most frequently cited and trusted fluorochromes, with the kits to ensure the quality of the fluorescent signal produced by the kits.  The kits will be available globally through the TTP website and their authorised distributors, alternatively register at http://ttplabtech.com/landing/sol-r-reagents/ to apply for TTP Labtech’s early access program.

The new sol-R reagent kits will be released at MipTec 2017, Basel, 11-13th September – customers should visit booth #A26 to find out more.

  1. Alexa Fluor 488 (AF488) Streptavidin, licensed by Thermo Fisher Scientific.


For further information please contact:
Zyme Communications
Lorna Cuddon
Tel: +44 (0)7811 996 942
Email: lorna.cuddon@zymecommunications.com


For Abcam Media enquires please contact:
Instinctif Partners
Dr. Priya Kalia / Dr. Lynne Trowbridge
T: +44 (0)20 7866 7862 / +44 (0)20 7457 2861
E: abcam@instinctif.com

 

About TTP Labtech http://ttplabtech.com/

TTP Labtech designs and manufactures robust, reliable and easy-to-use solutions for sample management, liquid handling and multiplexed detection in drug discovery and genomics. We enable life scientists, through collaboration, deep application knowledge and leading engineering, to accelerate research and make a difference together. Our essential tools include state-of-the-art solutions developed for high throughput compound and biologics screening (acumen® Cellista, mirrorball® and fully validated consumables such as sol-R™ beads and plates),flexible sample management workflows from ambient to -800C (comPOUND®, arktic®, lab2lab), and unique low-volume liquid handling for genomics, compound screening and protein crystallography (mosquito® X1, mosquito® HTS, mosquito® crystal, mosquito® LCP, mosquito® HV, dragonfly® crystal and dragonfly® discovery and a full range of validated consumables such as tips and plates).


About Abcam plc www.abcam.com

As an innovator in reagents and tools, Abcam’s purpose is to serve life science researchers globally to achieve their mission, faster. Providing the research and clinical communities with tools and scientific support, Abcam offers highly validated biological binders and assays to address important targets in critical biological pathways.

Already a pioneer in data sharing and ecommerce in the life sciences, Abcam’s ambition is to be the most influential company in life sciences by helping advance global understanding of biology and causes of disease, which, in turn, will drive new treatments and improved health. Two-thirds of the world’s 750,000 life science researchers use Abcam’s affinity binders, reagents, biomarkers, and assays, and the company’s products are mentioned in over 20,000 of the 56,000 peer-reviewed papers published each year in the life sciences.

By actively listening to and collaborating with researchers, Abcam continuously advances its portfolio to address their needs. A transparent programme of customer reviews and datasheets, combined with an industry-leading validation initiative, gives researchers increased confidence in their results.

Abcam’s twelve locations are located in the world’s leading life science research hubs, enabling local services and multi-language support. Founded in 1998 and headquartered in Cambridge, UK, the company sells to more than 100 countries. Abcam was admitted to AIM in 2005 (AIM: ABC).

 

3 - 7 Feb 2018 Visit us at SLAS2018, booth 1219

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Join us at SLAS2018!

We will be showcasing solutions for sample management, liquid handling and detection instrumentation which are ideally suited for use in:

Target discovery  – simple answers from complex biology

  • arktic – biological library -80°C automated storage
  • mosquito – low-cost, low-volume NGS library prep
  • acumen Cellista – genome-wide library profiling
  • comPOUND – rapid automated delivery from -20°C storage

Assay development – develop complex assays, faster

  • dragonfly discovery – automated multi-channel assay development and validation for HTS
  • mosquito – low-volume compound addition and dilution
  • acumen Cellista – high-content phenotypic screens
  • mirrorball –no-wash, multi-target immunoassays

High-throughput screening – robust and cost effective screening

  • dragonfly discovery – robust, accurate and reliable HTS dispensing
  • mosquito – simple assay ready plate creation and reformatting
  • acumen Cellista – practical uHTS phenotypic screening
  • mirrorball – multiplexed, no-wash immunoassays
  • comPOUND – rapid automated delivery from -20°C storage

Hit-to-lead – progress with confidence

  • dragonfly discovery –  complex assay setup and screening
  • mosquito – low-volume hit picking and serial dilutions
  • acumen Cellista – multiplexed cell heath, multi-parameter 3D assays
  • mirrorball – multiplexed cell-based hit characterisation
  • comPOUND – compact, high-speed cherry picking -20°C storage

For more information about SLAS2018 please visit their website here

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3 - 4 Oct 2017 Coming to ELRIG Drug Discovery 2017?

We hope you will be at ELRIG Drug Discovery 2017, as we’ve got a surprise!

Venue: ACC, Liverpool, UK
Date: 3 – 4 Oct 2017
Talk: TBC
Title: TBC
Day:
TBC
Time: TBC

ELRIG’s flagship event for drug discovery is back for its 11th year and we will be there for a talk session.

The conference explores innovative technologies and approaches in the world of drug development. With a series of workshops, presentations and talks we hope you will attend, and come to our talk!

Check back here soon for more information!

For ELRIG Drug Discovery information please click here

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29 Aug - 1 Sept 2017 Join us at Genome 10K and Genome Science conference 2017

Meet mosquito at this years Genome 10K and Genome Science conference

Venue: Earlham Institute, Norwich, UK
Date: 29 Aug – 1 Sept 2017
Presentation: 31 Aug 2017, 9:45am, Session 6b, Klaus Hentrich (TTP Labtech)
Automated low-volume liquid handling for cost-effective NGS library preparation and single cell genomics

Join the team at this years Genome 10K and Genome Science conference, hosted at the Earlham Institute in Norwich.

We will be showcasing our liquid handling instrument mosquito!

Unlike traditional liquid handlers, the mosquito robot uses  positive displacement liquid handling technology. This superior pipetting mechanism allows dispensing in the 25 nL – 5 µL range with high precision and accuracy, across the volume range. Using this technology to prepare lower reaction volumes results in dramatic cost savings while preserving data quality compared to standard reaction volumes.

Furthermore, the use of disposable mosquito tips ensures zero cross-contamination and gentle sample handling. Additionally, it offers the ability to pipette liquids with high viscosities (such as enzymes in 50% glycerol or genomic DNA) accurately and precisely. It provides a unique opportunity to maximise data output in a Genomics research laboratory or core facility setting.

We hope to see you at Klaus Hentrich (TTPLabtech) presentation:
Automated low-volume liquid handling for cost-effective NGS library preparation and single cell genomics

Date: 31 Aug 2017
Time: 9:45am
Where: Session 6b

For more information about the Genome 10K and Genome Science conference please click here

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app note: comPOUND & arktic storage and cold sorting of clinical research samples in the laboratory for maximum sample integrity

blog: the perfect couple for GPCR

blog: the perfect couple for GPCR.

Obtaining detailed structures of any membrane protein has been challenging. A detergent is required to extract the protein from the lipid environment which does not contribute to a favourable environment for crystallisation using traditional vapour diffusion methods. To overcome this problem the lipid cubic phase (LCP) crystallisation method was developed. LCP was originally limited only by the difficulties associated with handling and pipetting the extremely viscous cubic phase (monoolein and water) a hurdle that has now been overcome by the development of TTP Labtech’s robotic system, mosquito® LCP.

Crystallography has clearly made many contributions to science over the last century. The ongoing process of developing new therapeutic drugs has driven research towards a desire to understand drug-target interactions at a molecular level. One group of potential targets attracting a great deal of attention is the G-protein coupled receptors (GPCRs). GPCRs are a large and diverse group of eukaryotic membrane receptors that play a role in a plethora of biological functions from olfaction to mood regulation.

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mosquito LCP

Many drugs exert their effects by binding to GPCRs and thus understanding the structure of this interaction is of great importance.

The use of mosquito LCP has alleviated many of the technical limitations around the crystallisation of membrane proteins and allowed the crystallography research community to successfully determine many new GPCR structures that relate to drug therapy. Below are a few examples of the these which have all used mosquito LCP as an essential tool in determining the structures.

anxiety and depression

Research at Heptares Therapeutics has resulted in several publications in Nature describing the crystal structures of class B and class C GPCRs. Corticotropin-releasing factor receptor type 1 (CRF1R) is a class B GPCR involved in mediating the body’s response to stress and has thus been a target of drugs designed to treat both anxiety and depression. This was the first crystal structure of a class B GPCR to be determined1. More recent work from Heptares has determined the crystal structure of a class C GPCR, metabotropic glutamate receptor 5 transmembrane domain (mGlu5). mGlu5 responds to the neurotransmitter glutamate, which may open up research into the treatment of fragile X syndrome, autism, depression, anxiety, addiction and movement disorders2.

type 2 diabetes

Type 2 diabetes affects over 3 million people in the UK alone and is the result of an insufficient insulin generation leading to higher than normal levels of blood glucose. A specific GPCR known as the human GPR40 receptor (hGPR40) has been shown to enhance glucose-dependent insulin secretion. Recent work has led to the development of TAK-875, a selective agonist of hGPR40 from Takeda that reached Phase III trials for the treatment of type 2 diabtes3. Crystallographic methods, involving the use of automated robotic systems (including mosquito), successfully described novel hGPR40-TAK-875 interactions4.

tackling blood clots

Recent work in crystallography has looked at a particular type of GPCR known as the P2Y12 receptor (P2Y12R), which is part of a family making up one of the most common targets of drugs that work to inhibit platelet aggregation5. Platelets play a critical role in thrombus formation (blood clotting), and drugs targeting P2Y12R have been approved for the prevention of stroke and myocardial infarction. Studies by Zhang et al revealed exciting and surprising structural findings:

P2Y12R was found to be capable of undergoing striking extracellular conformational changes akin to an open or closed ‘binding pocket’ depending on the type of agonist of antagonist present.

the integration of automation

Automated pipettors such as the mosquito range (TTP Labtech), used in all of the research detailed here, have been described as ‘the iPads of robotics’ by a recent user. Technology has allowed many users to progress from manually setting up crystallography screens and conducting multiple experiments in 24-well plates to rapidly seeding a 96-well screen. In the process the added advantages are the use of very low volumes of reagents with varying viscosities and, with a high degree of both accuracy and precision. These features have proven essential in taking the challenge of crystallising membrane proteins to a relatively routine procedure.

mastering the black art

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LCP dispensing using mosquito LCP

Using these new systems has meant that even proteins that have been notoriously difficult to crystallise due to their complex structure, poor availability or unique characteristics, can now be studied. Whether using hanging drop, microbatch, vapour diffusion, sitting drop or lipid cubic phase (LCP) set-ups, there are now systems available to help alleviate many of the experimental hurdles experienced in the past.

For the full article please go to http://www.labnews.co.uk/features/the-perfect-couple/. If you would like any further information about any of the liquid handling mosquito or dragonfly® systems please contact crystallography@ttplabtech.com.

References

  1. Hollenstein, K. et al. Structure of class B GPCR corticotropin-releasing factor receptor 1. Nature 499, 438–43 (2013).
  2. Doré, A. S. et al. Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain. Nature (2014). doi:10.1038/nature13396
  3. Negoro, N. et al. Discovery of TAK-875: A potent, selective, and orally bioavailable GPR40 agonist. ACS Med. Chem. Lett. 1, 290–294 (2010).
  4. Srivastava, A. et al. High-resolution structure of the human GPR40 receptor bound to allosteric agonist TAK-875. Nature (2014). doi:10.1038/nature13494
  5. Zhang, J. et al. Agonist-bound structure of the human P2Y12 receptor. Nature 509, 119–22 (2014).