Multiplex assays, which measure several biological parameters in a single study, are a popular method for the simultaneous analysis of parameters such as cellular activity, cell surface markers, phenotypes and toxicity in compound screening. The ability to capture data on two or more parameters at one time point on the same cells increases throughput, minimises cell culture and consumable requirements, thereby reducing time and cost.
Multiplexing can save time and be more cost efficient than parallel assays on different samples.
TTP Labtech’s acumen® Cellista high content imager can be successfully employed for a wide range of cell-based multiplex assays. From a single scan, acumen offers rapid and simultaneous data capture of a range of parameters such as enzyme inhibition, plasma membrane integrity, cell viability, proliferation, cell cycle analysis and apoptosis. In addition, acumen Cellista can be used for analysis of 3D cultures, tissues or even small organisms due to its large depth of field. acumen’s Cellista software offers better data handling, faster scan times and open source TIFF export, enabling the provision of data rich results in a time and cost efficient manner.
TTP Labtech’s mirrorball® is the first system in its class to offer simultaneous scanning with multiple lasers. A common approach for antibody discovery is the screening of libraries against parental and transfected cell lines to identify antigen-specific binding. Normally, this requires the running of separate assays for each cell line, however, mirrorball’s multiplexing capabilities allows tests to be performed in a single well. This has several advantages including elimination of inter-plate variability, increased throughput and reduced reagent usage.