liquid handling for hanging drop creation

Hanging drop vapour diffusion – where a combined drop of protein and screen is suspended over the well – is commonly used in high-throughput protein crystallisation screening because:

  • it exactly replicates the manual method, without introducing new experimental variables
  • crystals grown are not stuck to the bottom of the plate, and thus hard to remove

TTP Labtech’s mosquito has the ability to automatically ‘mirror’ drops of crystal screen on a plate seal, so when the seal is placed over the plate, the drops match the screen wells. Precision placement and accurate pipetting mean that drop volumes can be miniaturised without the risk of the different components not coinciding. In fact, placement is so accurate that multiple drops can be created in each well of a 96-well plate, allowing you to test up to 288 different conditions per plate.

>> Watch our tutorial on creating multiple hanging drops per well 

mosquito Crystal has been an enabler for Otago University, making it possible to work with more proteins that are only available in small amounts. This allows new projects to be started more readily, thus opening new vistas for scientific inquiry.

>> Read the University of Otago case study in our labcrystal magazine

one robot for many protocols

Easily swap between hanging and sitting drop creation thanks to mosquito’s simple protocols. There is no need to make physical changes to the robot setup. Disposable positive displacement micropipettes easily carry out additions down to 10 nL of even heavy metals or viscous liquids with zero cross-contamination.

>> Download our ‘automation of protein crystallisation drop set-up’ poster

safe seals and plates for hanging drops

TTP Labtech offers a range of high performance, ultra-clear plate seals specifically designed for hanging drop protein crystallography, with options suitable for UV or non-UV imaging.

>> View TTP Labtech’s specialist plate seals

TTP Labtech hanging drop plates have a void space around the wells, which makes cutting around the outside edge of a well and then peeling the piece much easier compared to other plates.

>> View TTP Labtech’s specialist crystallisation plates

what do mosquito users say?

“I use the mosquito for coarse screening and fine screening because of the minimal protein it uses and the fact that I can set-up hanging drops as well as sitting drops.”
Heather Stone, Takeda, on SelectScience

>> Visit the SelectScience website for independent mosquito Crystal reviews

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