Many commonly used plate reader assays (e.g. ATPluminescence measurements) assume
a linear relationship between the assay signal, the number of cells and their viability. However, as this application note illustrates, this assumption is not always justified: ATP-luminescence measurements can significantly overestimate toxicity and underestimate potency, leading to false-negative viability/ efficacy hits.
This application note shows the development and implementation of an alternative no-wash high content (HCA) assay using acumen, which eliminates common problems associated with ATP-luminescence measurements. We compare the drug responses of several well-characterised anti-cancer drugs on HeLa cells, measured by acumen and a commercial ATP-luminescence detection system, and highlight key differences between the measurements.
>> Read the full application note here