acumen: siRNA screening – from sample storage to phenotypic results

Typically siRNA screening programs are run using pooled sets of at least three siRNAs that are supplied in either a 96- or 384-well plate format and screened in a variety of ways. Hits are identified as positive results, then the individual siRNAs from that pool are re-transfected as single siRNAs and tested again to determine which siRNA elicited the effect.

This approach faces several technical challenges.

Here we present an integrated siRNA screening solution from TTP Labtech which overcomes these challenges and enable robust and fast genome-wide phenotypic screens.