The use of monoclonal antibodies against a wide range of protein targets is an expanding area for the development of therapies and biomarkers of diseases such as cancer and autoimmune disorders. Monoclonal antibodies are produced by cell lines that secrete antibodies against a specific antigen into the growth medium. High-throughput screening is central to antibody discovery programmes due to the large number of clones that need to be assessed.
This application note describes the use of TTP Labtech’s mirrorball for the determination of antibody binding to human epidermal growth factor receptor (EGFR) using a “mix-and-read” assay as an example of a screen for a cell surface antigen.
In this application note, we demonstrate that mirrorball is capable of distinguishing antibody binding in more than one cell line in a single well. This ability to multiplex reduces costs, increases throughput and eliminates intraplate variability.