In this application note, we demonstrate the transfer of standard commercially available colorimetric IL-8 and IL-6 ELISA kits onto a no-wash fluorescence based ELISA on the mirrorball. The resulting ELISA format offers significant process efficiency improvements through use of a single incubation step, without the requirement for wash steps, and is amenable to multiplexing.
Although ELISAs are very sensitive, they are time-consuming, require multiple separate wash steps and incubation steps and are not amenable to multiplexing.
TTP Labtech’s mirrorball facilitates transfer of ELISA to no-wash fluorescent ELISA assay format resulting in thoughput and automation advantages:
- single incubation step and no-washes required
- smaller sample volume required due to assay miniaturisation
- multiplexing capability gives more information per sample
- overall process efficiency improvement freeing up valuable time and sample for other tests