mosquito HTS: Nextera XT library preparation and magnetic bead clean up

This application note presents a high-throughput, miniaturised workflow, in which differentiated pancreatic stems cells were studied using single-cell RNA-Seq (scRNA-Seq), in Dr. Louise Laurent’s lab at University of California, San Diego, USA.

Miniaturisation of the library prep volumes was achieved using TTP Labtech’s mosquito HTS and HV liquid handlers, which accurately dispense volumes of between 25 nL and 1.2 μL and 0.5 to 5 μL, respectively.

mosquito liquid handlers for low-volume single-cell RNA-Seq library preps:

  • reduce cost through miniaturisation of reagent volumes
  • provide reliable pipetting of solutions with low to high viscosity
  • increase reproducibility and data quality with accurate pipetting at low volumes