Compound screening requires the serial dilution of lead compounds at all stages of development from early secondary screening to toxicity studies. Most of these assays are high throughput, using automation with minimal sample volumes to save time and money, but there are a number of limitations associated with doing this. Some of these include achieving high accuracy and precision, avoiding large dead volumes, ability to aspirate and mix, flexibility to use different plate formats and to perform different experimental set-ups.
TTP Labtech’s automated liquid handling range overcomes all of these issues since it is based on true positive displacement. The mosquito HV is extremely accurate within the 500 nL to 5 µL volume range across a wide range of liquid viscosities, in 96-, 384- and 1536-well plate formats.
A serial dilution wizard in the mosquito software allows the user to very quickly and easily configure, generate and save a serial dilution protocol. Standard protocols like ‘assay-ready’ dilutions, mother/daughter plate replication, or plate reformatting are all very easy to perform on mosquito and do not require set-up changes to the instrument. This sort of process flexibility is essential to high throughput screening labs.
This application note explains how serial dilution assays can be achieved using the mosquito HV serial dilutions wizard and the results from three case study examples.