mirrorball® high sensitivity cytometer for antibody discovery
For cell based assays, TTP Labtech’s mirrorball® high sensitivity cytometer represents one of the most versatile instruments available today.
Traditional techniques for antibody analysis, such as ELISA and flow cytometry, can represent a bottleneck in the antibody discovery process as they require lengthy, multi-step preparation stages, large cell numbers and amounts of costly reagents. The simplified protocol of a mix-and-read assay or homogenous no-wash assays employing fluorometric microvolume technology (FMAT®) speed up the process of antibody discovery by providing improved data quality and decreased sample and reagent usage. It is particularly beneficial in the study of high cost or low abundance antigens.
mirrorball’s flexibility allows direct transfer of cell based assays that normally require ABI 8200 (FMAT®), high content imaging or flow cytometry. It is the first system in its class to offer simultaneous scanning with multiple lasers (405, 488 and 640 nm) and offers a unique set of enabling features:
- eliminating wash procedures yet retaining high sensitivity, mirrorball is capable of performing cell based assays compatible with both adherant and non-adherent cell lines.
high speed multiplexing
- for identification of multiple fluorophores and analysis of heterogeneous populations in bead and cell based assays.
high content imaging
- with whole well scanning, mirrorball requires fewer cells to achieve statistically significant results.
flow cytometry in a microplate
- with high sensitivity, mirroball offers an alternative to flow cytometry for the detection of low affinity antibodies and proteins found in low abundance.