sol-R coded beads: making light work of ELISA

TTP Labtech’s coded beads represent a range of reagents for the quantification of secreted protein in multiplexed screening assays. Designed for use with TTP Labtech’s mirrorball fluorescence cytometer, sol-R™ beads are set to revolutionise the immunoassay workflow.

  • up to 5 multiplexable bead codes
  • no-wash, mix-and-read protocol
  • screening-friendly price point

There are many reasons why gold standard immunoassays are not widely utilised within a screening environment including: multi-step protocols that are complex to automate, restricted throughput, limited multiplexing and high screening costs.

Remove these limitations with TTP Labtech’s sol-R coded beads and watch your costs go down and your productivity go up.

4_points_mirrorball

sol-R streptavidin (SA) coded beads

TTP Labtech’s sol-R SA colour-coded multiplexed beads exploit the high affinity interaction between streptavidin and biotin for immobilizing biotinylated antibodies, or target proteins.

A typical assay workflow for sol-R SA beads begins with the separate immobilisation of individual biotinylated targets to individual bead codes. Individual codes are subsequently combined, then dispensed into an assay plate with detection reagent and sample.

bead preparation

bead_prep

sol-R™ no-wash screening protocol

sol-R_no_wash_screening_pro

example assay: antibody screening

For antibody screening, multiplexing of target specificity and selectivity binding protocols enhances productivity through the combination of 3 assays into 1, saving time, consumable costs and conserving precious sample (just 10 μL required for 384-well assays).

example_Assey

sol-R carboxy (COOH) coded beads

A typical sol-R COOH bead workflow begins with the separate covalent coupling of individual antibodies or target proteins onto individual bead codes using simple carbodiimide coupling chemistry. Coated beads are stable and therefore may be prepared in sufficient quantities to supply your entire screening campaign. Individual codes are subsequently combined, then dispensed into an assay plate with detection reagent and sample, or stored for future use in subsequent screening runs.

example assay: cytokine quantification

sol_r_carboxy_beads

Combination of anti-IL6, anti-IL8 and blank beads in a no-wash assay format offers a significant process improvement, versus traditional ELISA for consumable cost savings and protocol step reductions that result in free time and budget for additional tasks.

bead preparation

sol_r_carboxy_beads_2

available sol-R™ kits

Any combination of codes 1-5 may be brought together into sol-R™ bead kits. Kits are optimised for 384-well plates but protocols for 96-well and 1536-well plates are also available. TTP Labtech’s sol-R beads may be purchased in volumes for dispensing into 1, 5, 20, 50 & 100 assay plates.

Contact sales@ttplabtech.com for pricing enquiries, MSDS or for more information.

>> Download the TTPLabtech sol-R flyer

related brochures