key technical features

mirrorball®

main application Target based biologics screening
laser excitation Choice of 405, 488 or 640 nm
detection Photomultiplier tube (simultaneous)
fluorescence detection 2, 4 or 5 channels  plus laser scatter
scan area Whole well as standard
open source TIFF export Yes, with no compromise on speed
plate compatibility 96, 384 and 1536

 

plate format 96-well 384-well 1536-well
plate read time (mins)* 12 12 12
plates per 24 h 115 115 115
wells per 24 h 11,355 45,420 181,680
total data for 24 h operation 9 Mb 30 Mb 100 Mb

* acquisition plus analysis times for mirrorball obtaining whole well data

automation

Transfer of ELISA and ABI 8200 protocols has proved straightforward and required minimal development to run on a mirrorball.

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mirrorball’s proprietary optics allow determination of antibody-antigen interaction in the presence of a fluorescent tracer. This means that such interactions can be studied at equilibrium so that antibodies with low affinity or high rates of dissociation are not missed, as is the case for ELISA and flow cytometry.

 

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mirrorball’s specification includes triple laser excitation and five colour fluorescence detection enabling analysis of multiple antigens or cell types simultaneously.

 

 

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Microplate-based cytometric analysis is an ideal solution for bead and cell-based immunoassays. Adherent and suspension cell lines can be addressed in situ thus circumventing the many issues seen with flow cytometry such as clogging, cytotoxicity, and prolonged sample preparation.