mirrorball immunoassays

mirrorball-header

Immunoassays have been, and are still, an essential tool during many stages of the drug discovery process, but these assays can become a bottleneck due to limited throughput and capability. ELISAs are simple and inexpensive but can only detect one target thereby numerous individual assays are required to measure multiple targets. Newer approaches address some of these limitations through multiplexing or homogeneous assay formats; however, these often result in greatly increased reagent and consumable costs. Many are limited in flexibility to just immunoassays, whereas more versatile systems such as flow cytometry are generally less suited to a screening environment.

Meet the alternative – mirrorball, a highly sensitive fluorescence cytometer!

no-wash screening protocol

A simple two-step addition process is all that is required for bead or cell-based
homogeneous, multiplexed immunoassays

no-wash

screening and characterisation in a single assay

Delivering data-driven decisions faster and within budget

example assay:
binding to a cell surface target

3-plex

Here we present a simple homogenous (no-wash) assay to screen antibody samples for binding to a target cell line, non-specific binding to a control cell line and antibody titre by binding to a bead. Such a set-up allows clear distinction between affinity and titre, which is easily obscured in singleplex experiments.

example assay:
bead-based cytokine quantification

antibody-assay

A combination of interleukin antibodies and blank beads in a no-wash assay format offers a significant process improvement, versus traditional ELISA for consumable cost savings and protocol step reductions that result in free time and budget for additional tasks.

example assay:
bead-based antibody screening

antibody-assay

For antibody screening, multiplexing of target specificity and selectivity binding protocols enhances productivity through the combination of 3 assays into one well, saving time, consumable costs and conserving precious sample (just 10 μL required for 384-well assays).

Would you like to speak to one of our specialists to discuss your personal workflows and how we can help? Would you like to see mirrorball and the no-wash assays in action? We’re here to help!

cell and bead based screening applications

mirrorball versus ELISA mirrorball versus flow cytometry
no-wash protocols minimise hands-on time for cell- and bead-based immunoassays
multiplex up to 5 targets to save costs and time in-situ adherent cell analysis eliminates cell suspension protocol step
conserve precious sample (10 μL / test) for downstream hit characterisation significantly decreased operational and maintenance costs
hit characterisation through image-based functional assays non-fluidics approach: no delays from system clogging; no potential carry-over
faster plate read times (12 min/plate)
whole well images permit visual confirmation of results for assured decision-making
automation-friendly workflows increase throughput capacity