protein crystallisation with mosquito LCP

TTP Labtech’s mosquito LCP overcomes the common problems encountered with accurately dispensing the highly viscous LCP mixture used in membrane protein crystallisation. It allows you to fully automate LCP set-ups accurately and repeatedly.

automated lipid cubic phase screening

mosquito LCP allows you to dispense lipidic cubic phase (LCP) volumes as low as 25 nL, while automated calibration of syringe and pipette positioning ensures precise drop-on-drop placement for easy automated imaging.

In addition, mosquito LCP includes all the functionality of mosquito Crystal for your automated vapour diffusion set-ups. This allows you to set up both LCP and traditional protein crystallisation experiments in any commercially available plate, using just one instrument, with no configuration changes or additional hardware or software.

mosquito LCP employs TTP Labtech’s proven positive displacement mosquito tips for screen additions offering guaranteed zero cross-contamination.

TTP Labtech also offers additional components to enhance your membrane protein crystallisation screening, such as a humidity cover and an automated mixer for preparing LCP.

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>>Optimising automated low volume, high value protein crystallisation screening

 

mosquito® accessories & spares

  • plate seal holder for hanging drop preparations
  • calibration block for optimising mosquito set up
  • guide base for positioning  hanging drop seal onto reservoir plate
  • pipette tape spool cover to protect tips from environmental  particles
  • 4-position ultra low reagent holder to conserve valuable sample

mosquito® LCP syringe coupling

A knurled metal coupling for joining two Hamilton Gastight® Syringes, with very low dead volume, used in mixing the protein and monoolein to prepare the LCP mixture, either using TTP Labtech’s LCP mixer or manually

mosquito® LCP needles (3/pack)

A short, precisely cut, low dead volume needle compatible with the 50 μL or 100 μL  Hamilton Gastight® Syringes used by mosquito LCP.

LCP mixer

The LCP mixer has been designed to automatically mix protein and monooelin (MO) or other lipids into a lipidic cubic phase from two coupled syringes. It accepts any combination of 100 µL and 50 µL Hamilton Gastight syringes and any starting sample volume (high or low) in either syringe. The simple and robust design, offers easy loading and ‘on the fly’ adjustments of mix cycles without the need for a full calibration process. The mixer has a compact footprint of only 250 mm x 214 mm, saving vital bench space.

active humidity chamber

Sample evaporation can be a problem when dispensing very low volumes or volatile samples, causing inconsistent drop sizes, especially when environmental conditions and local humidity vary.

mosquito’s active humidity chamber reduces experimental inconsistencies caused by variation in the humidity in the environment, by offering the offering the following benefits:

  • accurate control of the relative humidity (RH) of each experiment
  • enables up to a 90% reduction in drop evaporation
  • it takes only a few minutes to reach high levels of humidity (80-90% RH)
  • precisely controlled form within mosquito software (version permitting)

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mist rolling over the plate in the chamber

mist rolling over the plate in the chamber

If you would like more information or to order any of the accessories found on this page, please email sales@ttplabtech.com.

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