applications

TTP Labtech’s mosquito HTS liquid handling robot can be used for miniaturising a wide range of applications which require a volume range of 25 nL – 1.2 µL, including:

  • compound screening and hit picking
  • serial dilutions for determination of dose response curves
  • plate reformatting, including transfer between different plates types
  • plate replication
  • acoustic source plate creation
  • molecular biology based applications such as: next generation sequencing, DNA, RNA extraction and PCR/qPCR preparation
  • high-throughput MALDI-TOF MS
  • processing in protein precipitation
  • cell culture seeding and handling

serial dilutions

TTP Labtech’s mosquito HTS can create 500 nL to 2 μL total volume serial dilutions, automatically dispensing at high speed. For example, an 11 point low volume dilution series can be dispensed in 1.5 minutes.

>> Poster: Miniaturized serial dilution of compounds: essential for ultrahigh throughput screening (uHTS) drug discovery

plate reformatting and replication

TTP Labtech’ s mosquito HTS liquid handling robot enables easy and accurate reformatting between 96-, 384- and 1536-well plates, including replicating mother plates directly into high-density, low-volume daughter plates within 1.5 mins.

acoustic source plate creation

Fast plate set-up, excellent sample recovery and integrated plate seal piercing makes mosquito HTS a very efficient tool for acoustic source plate creation.

next generation sequencing

TTP Labtech’s mosquito HTS offers an accurate and easy to use solution which can be applied to miniaturising next generation sequencing (NGS) reaction volumes within an automated, high throughput setting. Additionally, it offers ability to pipette liquids with high viscosities (such as enzymes in 50% glycerol) accurately and precisely with no cross contamination. It provides a unique opportunity to obtain the current level of data at a significantly lower cost, and/or to carry out larger scale studies at current costs.

Featured in JALA with Louise Laurent’s lab (UCSD): Miniaturization Technologies for Efficient Single-Cell Library Preparation for Next-Generation Sequencing publication

Different stages of human pancreatic stem cells were studies using single cell RNA-seq (scRNA-seq). 2, 4 and 8 uL Nextera XT libraries were prepared for technical and biological replicates and using mosquito. The libraries were also cleaned up in 384 format on mosquito. The data confirms that miniaturization of reaction volumes does not affect the scRNA-seq data quality. >> Read publication!

Featured in Cell by Irving L. Weissman’s lab (Stanford Medicine): Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types

As previously sown in JALA paper, miniaturisation of reaction volumes does not affect the scRNA-seq data quality. Here high throughput scRNA-seq was used to systematically catalogue the diversity of intermediate cell states formed during differentiation, and the minimal combinations of positive and negative signals that were sufficient for differentiation between each of these intermediate states was tested. mosquito X1 was used for the normalisation of cDNA concentrations, and mosquito HTS was used for Nextera XT library prep at 4 uL. >> Read publication!

>> Poster: Methods for reduced cost and lower sample prep volumes for genetic analysis applications

>> Poster: Miniaturization technologies for cost effective library preparations for next-generation sequencing (NGS)

genotyping

PCR and qPCR is expensive. By miniaturising the assay, TTP Labtech’s mosquito nanolitre liquid handling capabilities can reduce the reagent cost of real-time PCR and SNP genotyping by up to 90%. In addition mosquito’s disposable micropipettes have the added benefit of eliminating cross-contamination.

high-throughput MALDI-TOF MS

mosquito HTS has been integrated into the fastest, automated mass spectrometry system for biochemical screening, designed to accelerate all phases of drug discovery. The MALDI PharmaPulse incorporates Bruker Daltonics’ autoflex speed MALDI-TOF system with robotic sample preparation and movement provided by TTP Labtech and HighRes Biosolutions. The system offers true label-free detection of native substrates and products in functional biochemical screens.

mosquito HTS provides low volume, high precision liquid handling and pipetting compatible with 96, 384 and 1536 formats. mosquito’s unique positive displacement technology allows sample and matrix to be sequentially aspirated and simultaneously dispensed to produce uniform, homogeneous spots in the sub microliter range. Highly accurate pipetting and positioning allows spots to be set up in high density 1,536 arrays to enable fast and efficient analysis. The homogeneous nature of the spots mean the MALDI laser can always fire in the same location without compromising data quality/signal strength and is key to the overall throughput of the system. The rigid nature of the tips also allows them to pierce foil-sealed source plates, which is essential for the preservation of volatile matrix and wash solutions. mosquito HTS is capable of preparing a 1,536 MALDI plate in less than 1 second per well – keeping pace with  analysis time of the MALDI-TOF and maintaining overall throughput.

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