applications – cell based screening

TTP Labtech’s acumen Cellista offers a highly versatile tool for high throughput cell based screening.

acumen Cellista is ideally suited to phenotypic screening in areas such as cell health, 3D cell culture models, cell signalling and RNAi profiling.

adipogenesis phagocytosis
angiogenesis phospholipidosis
apoptosis proliferation
beta-lactamase protein kinase profiling
cell cycle analysis protein translocation
cell differentiation reporter gene activation
cell migration small organism imaging

  • C elegans
  • Drosophila
  • Zebrafish embryos
chemotaxis spheroid analysis
colony formation stem cell phenotyping
cytotoxicity steatosis
mitotic index tissue imaging

case study #1: cell health

acumen Cellista makes it easy to incorporate toxicity screening into the early phases of drug discovery to identify compounds with potential off-target toxicity effects.

  • fast scan times and instant results report biologically-relevant data at the throughputs needed for primary screening
  • multiple toxicity readouts give a wealth of results from every single test well
  • whole well analysis gives you the confidence of robust data, quality controlled for cell number per well
  • small (CSV format) files ensure you’re not generating bottlenecks in data handling and validation

These assays include:

  • live/dead – using standard dyes such as calcein-AM/PI or TOTO-3/TMRM.
    These combinations provide a more cost-effective approach to bulk read assays, such as CellTitre–Glo®, and offer the benefit of multiplexing with additional phenotypic assays to gain more high content data
  • apoptosis – annexin V, Caspase-3, nuclear condensation
  • genotox – phophohistone H3

case study #2: 3D cell models

acumen Cellista allows you to combine biologically-relevant 3D cell culture models with high throughput methods to improve the quality of your hits in applications such as cancer treatment research.

  • whole well imaging means all colonies are counted
  • colony volumes are determined without z-stacking
  • easily distinguish between colonies and small clusters of cells
  • robust data is obtained in a little as 5 minutes per plate

This represents a paradigm shift in how modern pharmaceutical labs are conducting their screening.

acumen has been successfully used to describe the formation of multicellular spheroids on:

  • ultra-low attachment plates (TTP Labtech application note)
  • hanging drop plates (Horman, S. et al. (2013), J Biomol Screen)
  • colonies in semi-solid agarose (Bowen, W. & Wylie, P. (2007), Clin Lab Med)

related app notes

related videos