key technical features of acumen’s laser scanning imaging cytometry
As a company, TTP Labtech has collaborated with cell biologists since 1997. This enabled us to spot a market opportunity early on: a need for a screening system that was as fast as a bulk reader but provides high content data.
Cellular resolution at high throughput is achieved by laser scanning excitation through a specialized f-theta lens and photomultiplier tube (PMT) signal detection. This design enables rapid whole-well imaging for up to 4 multiplexed colours over an area of 20 mm x 20 mm without re-focusing, and facilitates the imaging of 3D models due to its large depth of field. It also eliminates the potential for focussing errors as there is no reliance on laser- or image-based autofocussing methods.
Using the principles of cytometry, acumen simultaneously acquires and analyses every object within each well, immediately reporting object-level data from which cellular sub-classifications may be derived. Those cellular characteristics indicative of a hit are reported in only 5 minutes, even for 1536-well plates.
This cytometric approach is quick to learn and data output files are small, making it simple to integrate acumen into a screening workflow with little change to your existing infrastructure. There is no need for extra data storage, expert operators, or additional software development.
|high speed, high sensitivity laser scanning||ensures you don’t miss the next blockbuster as full library phenotypic or target-based screening is now a practical reality|
|wide ranging reagent choice through triple laser excitation||flexibility to choose reagents to suit your budget or easily transfer assays from other HCA readers|
|large depth of field from acumen’s widefield F-theta lens’||improve hit selection using biologically-relevant 3D models in simple HTS format: no z-stacking, no image stitching|
|flat focus from widefield F-theta lens||save time by eliminating the potential for focussing errors|
|whole well analysis as standard||in-built quality control, as acumen’s total object number per well measurement validates results as you go|
|whole well analysis as standard||eliminates the risk of missing a hit and cuts costs by reducing the total cell numbers required|
|5 minutes for data acquisition and analysis||immediate decision-making data without the potential bottlenecks of image analysis|
|5 minute data acquisition time – even for 1536||significantly increase daily throughputs by easily miniaturising assays to drive down reagent and cell cost per well|
|template-driven software with cytometric data analysis||suitable for HTS as labs can be productive straight away: no requirement for expert resources|
|small file sizes from cytometric data||increase throughput without creating a data mountain to manage|
|microplate format: 96- to 1536-well||freedom to work with any SBS-format clear bottom plate|
|OME-compliant TIFF files||rapidly export whole well images which can be analysed in your existing software of choice|
|simple automation interface||easily slots into your existing infrastructure with seamless hardware and data integration|