key technical features of acumen’s laser scanning imaging cytometry

As a company, TTP Labtech has collaborated with cell biologists since 1997. This enabled us to spot a market opportunity early on: a need for a screening system that was as fast as a bulk reader but provides high content data.

Cellular resolution at high throughput is achieved by laser scanning excitation through a specialized f-theta lens and photomultiplier tube (PMT) signal detection. This design enables rapid whole-well imaging for up to 4 multiplexed colours over an area of 20 mm x 20 mm without re-focusing, and facilitates the imaging of 3D models due to its large depth of field. It also eliminates the potential for focussing errors as there is no reliance on laser- or image-based autofocussing methods.

Using the principles of cytometry, acumen simultaneously acquires and analyses every object within each well, immediately reporting object-level data from which cellular sub-classifications may be derived. Those cellular characteristics indicative of a hit are reported in only 5 minutes, even for 1536-well plates.

This cytometric approach is quick to learn and data output files are small, making it simple to integrate acumen into a screening workflow with little change to your existing infrastructure. There is no need for extra data storage, expert operators, or additional software development.

feature benefit
high speed, high sensitivity laser scanning ensures you don’t miss the next blockbuster as full library phenotypic or target-based screening is now a practical reality
wide ranging reagent  choice through triple laser excitation flexibility to choose reagents to suit your budget or easily transfer assays from other HCA readers
large depth of field from acumen’s widefield F-theta lens’ improve hit selection using biologically-relevant 3D models in simple HTS format: no z-stacking, no image stitching
flat focus  from widefield F-theta lens save time by eliminating the potential for focussing errors
whole well analysis as standard in-built quality control, as acumen’s total object number per well measurement validates results as you go
whole well analysis as standard eliminates the risk of missing a hit and cuts costs by reducing the total cell numbers required
5 minutes for data acquisition and analysis immediate decision-making data without the potential bottlenecks of image analysis
5 minute data acquisition time – even for 1536 significantly increase daily throughputs by easily miniaturising assays to drive down reagent and cell cost per well
template-driven software with cytometric data analysis suitable for HTS as labs can be productive straight away: no requirement for expert resources
small file sizes from cytometric data increase throughput without creating a data mountain to manage
microplate format: 96- to 1536-well freedom to work with any SBS-format clear bottom plate
OME-compliant TIFF files rapidly export whole well images which can be analysed in your existing software of choice
simple automation interface easily slots into your existing infrastructure with seamless hardware and data integration