Fluorescence based assay methodologies are enabling and invaluable technologies for modern high throughput screening (HTS). However, the majority of fluorescence technologies are limited by a number of compound interferences, most notably; quenching, auto-fluorescence and light scattering. Fluorescence lifetime (FLT) technology is based on measuring the time a fluorophore spends in the excited state before returning to the ground state. FLT is an attractive readout parameter due to its ability to mitigate some of the aforementioned compound-related assay interferences, in addition to being insensitive to well-to-well volume variations and less prone to turbidity by precipitating particles. This poster demonstrates that the ameon reader is capable of detecting FLT in a 1536-well microplate with the same sensitivity as a 384-well microplate.