mirrorball: simultaneous determination of antibody binding, specificity and titre on the mirrorball fluorescence cytometer

In this poster we present a simple homogenous (no-wash) assay to screen antibody samples for binding to a target cell line, non-specific binding to a control cell line and antibody titre by binding to a bead. Such a set-up allows clear distinction between affinity and titre, which is easily obscured in singleplex experiments. The assay was set up in 384-well plates, then read and analysed using the mirrorball fluorescence cytometer.

Download: mirrorball_simultaneous_determination_antibody_binding_poster_Nov2016.pdf