In recent years developments in automation, miniaturisation and integration have made significant contributions to the membrane protein crystallisation process. Automation of protein crystallisation screening trials requires accurate placement of nanoliter volumes of protein and screen drops, in addition to the reproducible and accurate dispensing of solutions of varying viscosities.
This is particularly important for the set-up of the highly viscous lipid mesophases in the Lipidic Cubic Phase (LCP) crystallisation technique for membrane protein crystallisation trials. The use of liquid handling robots such as TTP Labtech’s mosquito® Crystal and mosquito® LCP has increased throughput and repeatability allowing for many more conditions to be screened. Although mosquito LCP can rapidly set up a crystallisation screen, the use of TTP Labtech’s active humidity chamber ensures there is minimal evaporation of the LCP drops which ultimately increases reproducibility.
This poster demonstrates the use of the active humidity chamber with mosquito LCP to increase the yield of GPCR crystals. The β1-adrenoceptor (β1AR) has been employed for this study.