The use of better sequencing reagents and powerful analysis software has reduced the cost of next-gen sequencing dramatically and even beaten Moore’s law in 2008. Moreover, in the past few years, the detection limits and throughput of analytical instruments, such as fragment analyzers and thermocyclers have significantly improved, allowing analysis of a lower amount of genetic material. However, one of the bottlenecks for high-throughput genomic applications has now significantly shifted towards the cost of sample preparation.
Miniaturizing the sample preparation volume provides the opportunity for significant cost savings and scaling down the required sample input to pg values.
This is especially essential for high-throughput applications, such as single-cell analysis or when the sample prep cost is significantly high.
In this poster we will present two case studies, in which the miniaturization of reaction volumes has reduced the cost of sample prep up to 5 times due to its accurate, true-positive displacement and cross-contamination free technology.