mosquito HV: automated liquid handling of low volumes for biological assay and screen set-up

The growth of functional genomics and proteomics has resulted in the identification of a rapidly increasing number of key targets involved in disease and disease mechanisms. The set-up of assays for the validation of targets and downstream screening has become difficult to perform accurately and efficiently employing manual liquid handling technologies.

Operations such as aliquot transfer from stock plates, serial dilutions, plate preparation and replication all require accurate and repeatable pipetting, as well as meticulous sample labelling, tracking and management. Furthermore, the handling of low volumes employed in miniaturised assays significantly increases the potential for liquid handling error. In order to ensure high quality laboratory results, it is paramount to maintain accuracy and precision during the set-up of complex assays. It is unrealistic to expect this sort of time-consuming assay preparation to be performed manually in a high throughput format.

TTP Labtech’s mosquito® HV has a dispensing range of 500 nL to 5 μL which successfully bridges the gap between nanolitre and microlitre transfer requirements. This enables the automated set-up of standard biological assays without the need for special labware or instrument configuration changes. mosquito HV offers extremely fast and accurate plate replication and serial dilutions. This makes it particularly applicable to workstation-based workflows. It is also capable of reformatting between different plate types in the same protocol – even from standard 96-well formats direct to high density 1536-well plates.

This poster demonstrates the successful employment of mosquito HV for the set-up of serial dilutions, assay miniaturisation and plate reformatting of both bead- and cell-based assays. It also confirms excellent cell viability post pipetting.

Download: mosquitoHV_biological-assay-screen-setup_TTPLabtech-poster-2013-portrait.pdf